One of the most important reagents used in clinical medicine and life sciences is antibodies. Although antibodies are widely used, there is no established protocol for how they should be validated prior to use. Many commercial antibodies are not fully validated before use. This can lead to unreplicable results or even project abandonment.
It can also result in substantial time, money, and sample loss. The establishment of standardization for antibody verification is therefore urgently needed. You can look for the best antibody validation facility online.
Image Source: Google
What is Antibody Validation?
The components of antibody validation include proving specificity (antibodies can distinguish between antigens), proving affinity, and finally, reproducibility. Despite this being a reasonable definition, there are still issues with the widespread implementation or application of validation standards.
The antibody supplier should have performed the task of checking that the antibody was indeed authentic. Although the seller is responsible to ensure the highest quality of the reagents, there are other factors that can affect the performance of antibodies. End-users must use the reagent in their own experiments.
For example, antibodies can change in transport because of improper storage at low temperatures. The end-user must also verify the antibody secondary to confirm its validity.
The researcher can reduce the risk of an experiment by taking small steps. The first is to be familiar with the product specification. This includes the validation for the application, the protocol, and the recommended dilution.
How Can Antibodies Be Validated?
You can also use different methods to validate antibodies for specific applications. These include immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA, immunoprecipitation, (Chip), and peptide array, etc.